Phase Contrast Microscopy: definition, parts, uses, working principle.

Phase Contrast Microscopy

What is phase contrast microscopy? A phase contrast microscopy converts slight differences in refractive index and cell density into easily detected variation in light intensity to observe living cells. This microscope is used for visualization of cell culture and live cells. Living cells can be observed without any staining. Unstained specimens have absorbed no light, … Read more

Confocal Microscope Principle, Uses, Parts, Advantages, and Disadvantages.

Confocal Microscope Principle, Uses, Parts, Advantages, and Disadvantages.

Confocal microscopy has various advantages over conventional optical microscopy, including a short depth of field, the elimination of out-of-focus glare, and the ability to collect optical slices serially from thick specimens. A prominent use of confocal microscopy in the biomedical sciences is the imaging of either fixed or live cells and tissues that have typically … Read more

Dissecting Microscope (Stereo Microscope) Definition, Uses, Parts, Principle.

Dissecting Microscope (Stereo Microscope) Definition, Uses, Parts, Principle.

Stereo microscopes, in contrast to compound light microscopes, often employ reflected illumination, or light reflected off an object’s surface, rather than transmitted (diascopic) illumination. When a specimen is too thick or opaque for compound microscopy, reflected light from the object enables for investigation. Unlike a compound microscope, transmitted illumination in most stereo microscopes is not … Read more

Types of Microscopes With Definitions, Principle, Uses, Labeled Diagrams

Types of Microscopes With Definitions, Principle, Uses, Labeled Diagrams

A microscope is a scientific instrument that is used to magnify the image of an object or sample in order to study its structure or features in detail. Microscopes are commonly used in scientific and medical research, as well as in education and other applications, to study small structures and organisms that are not visible … Read more

Agrobacterium-Mediated Gene Transfer – Mechanism, Applications, Advantages

Agrobacterium-Mediated Gene Transfer - Mechanism, Applications, Advantages

What is Agrobacterium? Agrobacterium-mediated Gene Transfer Factors affecting Agrobacterium-mediated Gene Transfer Agrobacterium-mediated gene transfer is a widely used technique for introducing foreign DNA into plants. However, successful gene transfer depends on several factors, including: Why transform plants using Agrobacterium? Agrobacterium-mediated gene transfer is a preferred method for transforming plants for several reasons: Overall, Agrobacterium-mediated gene transfer is a … Read more

Organic Waste Recycling – Definition, Characteristics, Methods, Steps, Significance

Organic Waste Recycling - Definition, Characteristics, Methods, Steps, Significance

Organic waste adds to greenhouse gas emissions, soil and water contamination, and other environmental problems. Composting, anaerobic digestion, and vermicomposting are among the methods that can be used to manage organic waste. What is Organic waste? Organic waste refers to any waste material that originates from living organisms or their byproducts. This waste can be … Read more

Microbial Degradation of Lignin – Microorganisms, Enzymes, Steps, Mechanisms, Challenges

Microbial Degradation of Lignin - Microorganisms, Enzymes, Steps, Mechanisms, Challenges

What is lignin? Structure of lignin Biological function of lignin Factors affecting lignin degradation The degradation of lignin is influenced by a variety of factors. Some of the important factors that affect lignin degradation are: Lignin-degradating Microorganisms The biological breakdown of lignin is an extremely crucial component of the intricate carbon and oxygen cycle that … Read more

Microbial degradation of cellulose – Enzymes, Steps, Mechanisms

Microbial degradation of cellulose - Enzymes, Steps, Mechanisms

What is cellulose? (C6H10O5)n Cellulose Molecular Weight/ Molar Mass 162.1406 g/mol Density 1.5 g/cm³ Appears White powder Melting Point 260–270 °C Properties of cellulose Structure of cellulose Commercial applications of cellulose What are cellulases? Factors affecting cellulose degradation Microorganisms involved in cellulose degradation/cellulolytic microorganisms Cellulose degradation is primarily carried out by cellulolytic microorganisms, which are … Read more

Phyllosphere Microorganisms – Examples, Factors, Effects

Phyllosphere Microorganisms - Examples, Factors, Effects

In microbiology, the phyllosphere is the complete above-ground plant surface seen as a microorganism habitat. Other subdivisions of the phyllosphere include the caulosphere (stems), phylloplane (leaves), anthosphere (flowers), and carposphere (roots) (fruits). The rhizosphere and laimosphere are the below-ground microbial habitats (i.e., the thin volume of soil surrounding root or subterranean stem surfaces). The majority … Read more

Soil Formation (Pedogenesis)- Definition, Factors, Process, Steps, Examples

Soil Formation (Pedogenesis)- Definition, Factors, Process, Steps, Examples

Definition of Soil Formation The process through which soil grows over time from weathered rock, organic matter, and other components in the environment is known as soil formation. This process involves the interaction of physical, chemical, and biological processes that transform basic materials into a complex, living environment that supports the growth of plants and … Read more

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