Rothera's test

Rothera’s test – Definition, Principle, Procedure, Result

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Published on August 31, 2024 10 min readby BNO Team

Acetoacetic acid and acetone react with an alkaline solution of sodium nitroprusside to form a purple-colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected.

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