Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Difference between One-step RT-qPCR and Two-step RT-qPCR

Difference between One-step RT-qPCR and Two-step RT-qPCR

In One-step RT-qPCR or One-step real time Polymerase chain reaction (PCR) the reverse transcriptase and DNA polymerase are premixed into a single tube.

Ad Blocker Detected
We've detected that you're using an ad blocker. Some content may not display properly.
Why are you seeing this? Ad blockers can prevent certain content and features from loading correctly on our website.

To continue with the best experience: