Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Pour Plate Method – Principle, Procedure, Objective, Result, Advantages

Pour Plate Method Principle, Procedure, Objective, Result

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more

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