Biotechnology

An example for template-independent DNA polymerase is (A) DNA Polymerase I (B) RNA polymerase (C) Terminal deoxynucleotidyl transferase (D) DNA polymerase III

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r): Assertion: Somatic embryogenesis in plants is a two-step process comprising of embryo initiation followed by embryo production. Reason: Embryo initiation is independent of the presence of 2,4-dichlorophenoxyacetic acid whereas embryo production requires a high concentration of 2,4-dichlorophenoxyacetic acid. (A) both (a) … Read more

Which one of the following DOES NOT belong to the domain of Bacteria? (A) Cyanobacteria (B) Proteobacteria (C) Bacteroids (D) Methanobacterium

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than 1 could be apparently due to (A) substrate inhibited kinetics with external pore diffusion limitation (B) internal pore diffusion limitation (C) sigmoidal kinetics (D) unstability of the enzyme

Differentiate between: (i) PCR and Gene Cloning. (ii) Batch Culture and Continuous Culture.

How is DNA sequencing performed using Sanger’s dideoxy technique? Give one limitation of this technique.

Explain how ‘Dolly’ was created. What is its importance in the field of biotechnology?

Write the names of any four important centres for biotechnology in India.

How are the diseases cystic fibrosis and albinism caused?

Mention the chief characteristics of embryonic stem cells. Give any two uses of such cells.