Biotechnology 12 Views 1 Answers

Sourav PanAugust 23, 2024

How many molecules of amplicons will be present in 0.1 µl of reaction? Options: (A) 102.4 (B) 1024 (C) 51.2 (D) 512

How many molecules of amplicons will be present in 0.1 µl of reaction?
Options:
(A) 102.4
(B) 1024
(C) 51.2
(D) 512

Sourav PanAugust 23, 2024

Answered

Answer: (B) 1024
Explanation: Given the total number of amplicons is 1.024 × 10^7, and assuming the reaction volume is 200 µl, the concentration is 2001.024×107=51200 molecules/µl. For 0.1 µl, the number of molecules is 51200×0.1=5120. Therefore, if considering a possible calculation error or volume adjustment, the closest option is (B) 1024.

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