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Consider the following statements. I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll. (A) only I is true (B) both I and II are true (C) only II is true (D) I is true and II is false
Consider the following statements.
I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase
II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll.
(A) only I is true
(B) both I and II are true
(C) only II is true
(D) I is true and II is false
Answer
Answer: (B) both I and II are true
T4 DNA ligase is known for its efficiency in blunt end ligation compared to E. coli DNA ligase, and its ligation efficiency can be improved with the addition of PEG and ficoll.
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