A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵
A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
(A) 4.50 × 10⁵
(B) 3.50 × 10⁵
(C) 4.20 × 10⁵
(D) 4.05 × 10⁵
Cite this post:
Sourav Pan. (2024, August 20). A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/
Sourav Pan. "A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵." Biology Notes Online, 20 August 2024, biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/.
Sourav Pan. "A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵." Biology Notes Online (blog). August 20, 2024. https://biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/.
Answer: (A) 4.50 × 10⁵
To have a 99% probability of finding at least one clone, one must consider the probability of not finding the clone and adjust accordingly. For a rare mRNA with 5 molecules per cell and assuming the probability of finding a clone in one screening attempt, approximately 4.50 × 10⁵ clones are needed to achieve this probability.
Cite this post:
Sourav Pan. (2024, August 20). A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/
Sourav Pan. "A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵." Biology Notes Online, 20 August 2024, biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/.
Sourav Pan. "A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ (C) 4.20 × 10⁵ (D) 4.05 × 10⁵." Biology Notes Online (blog). August 20, 2024. https://biologynotesonline.com/qa/a-cell-has-five-molecules-of-a-rare-mrna-each-cell-contains-4-x-10%e2%81%b5-mrna-molecules-how-many-clones-one-will-need-to-screen-to-have-99-probability-of-finding-at-least-one-recombinant-cdn/.
