Immunofluorescence Quenching Solution Preparation and Application

By BNO Team Published on May 14, 2024 1 min readby BNO Team

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Immunofluorescence Quenching Solution Application

Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.

Components required

The following components are required to prepare 1L of Immunofluorescence Quenching Solution.

Component	Amount	Concentration
Glycine (mw: 75.07 g/mol)	3.75 g	0.05 M
NH4Cl (mw: 53.49 g/mol)	2.67 g	0.05 M

Immunofluorescence Quenching Solution Preparation

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 3.75 g of Glycine to the solution.
3. Add 2.67 g of NH4Cl to the solution.
4. Add distilled water until volume is 1 L.
5. Quenching solution can be stored at ~20°C for several weeks.

References

“Immunofluorescence Quenching Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.

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