Vector – Definition, Examples, Characteristics, Types, Applications, Limitations

DNA Polymerase - Definition, Mechanism, Structure, Types

What is a vector in biology? Vector Definition A vector is a DNA molecule or genetic material used to transfer specific genetic information between cells in molecular biology and genetic engineering. Characteristics Features of vectors The following outlines the key features and functions of vectors, providing insights into their design and effectiveness. Types of vectors … Read more

DNA Polymerase – Definition, Mechanism, Structure, Types

DNA Polymerase - Definition, Mechanism, Structure, Types

What is DNA Polymerase? DNA polymerases Definition DNA polymerases are enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, playing a crucial role in DNA replication and repair across all living organisms. Properties of DNA Polymerase DNA polymerases possess several important properties that contribute to their role in DNA replication. These properties ensure … Read more

Next-Generation Sequencing (NGS)- Definition, Types, Applications, Limitations

Next-Generation Sequencing (NGS)- Definition, Types, Applications, Limitations

What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS), also known as high-throughput sequencing, has revolutionized the field of genomics and molecular biology by allowing the sequencing of thousands to millions of DNA molecules simultaneously. It encompasses a range of different sequencing technologies, all aimed at producing large amounts of sequence data at a lower cost … Read more

Mechanisms of Protein Synthesis Regulation in Eukaryotes Cells

Mechanisms of Protein Synthesis Regulation in Eukaryotes Cells

What is Protein Synthesis Regulation? Protein synthesis regulation refers to the various mechanisms by which cells control the production of proteins. Protein synthesis is the process by which cells generate proteins, which are essential molecules for the structure, function, and regulation of various cellular processes. The regulation of protein synthesis allows cells to adjust protein … Read more

DNA Ladders – Definition, Types, Uses

What is DNA Ladder? Definition of DNA Ladder A DNA ladder is a solution containing DNA molecules of known lengths that are used as a reference to estimate the size of unknown DNA fragments separated through gel electrophoresis. Characteristics of DNA Ladder DNA ladders used for electrophoresis possess several important characteristics to ensure their effectiveness … Read more

General Protocol for Western Blotting

General Protocol for Western Blotting

Principle of Western blotting The principle of Western blotting involves the separation of proteins based on their size through gel electrophoresis, followed by the transfer of the separated proteins onto a solid support (blotting), and the detection of the target protein using specific antibodies (detection). In the first step, gel electrophoresis, proteins are separated based on … Read more

Translational regulation – Definition, Mechanism

Translational regulation - Definition, Mechanism

What is Translational regulation? In conclusion, translational regulation is a vital process that controls protein synthesis levels by modulating the initiation, elongation, and termination stages of translation. It enables rapid cellular adjustments in response to various stimuli and is critical for maintaining cellular homeostasis. Understanding the mechanisms and factors involved in translational regulation contributes to … Read more

Transposable elements – Definition, Types, Examples, Application

Transposable elements - Definition, Types, Examples, Application

Barbara McClintock discovered the first transgenic elements in maize (Zea mays) at New York’s Cold Spring Harbor Laboratory. McClintock was conducting experiments on maize seedlings with broken chromosomes. McClintock planted self-pollinating corn kernels during the winter of 1944–1945, indicating that the silk (style) of the flower received pollen from its own anther.These kernels originated from … Read more

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