Restriction Enzyme (Restriction Endonuclease) – Definition, Types, Examples, Functions

Restriction Enzyme (Restriction Endonuclease) - Definition, Types, Examples, Functions

What is Restriction Enzyme (Restriction Endonuclease)? Definition of Restriction Enzyme (Restriction Endonuclease) A restriction enzyme, also known as a restriction endonuclease, is an enzyme that cuts DNA at specific recognition sites, called restriction sites. It is primarily found in bacteria and archaea and serves as a defense mechanism against invading viruses. Restriction enzymes are commonly … Read more

Types of RNA with Structure and Functions

Types of RNA with Structure and Functions

What is RNA? Different Types of RNA and Their Functions RNA, or Ribonucleic acid, is a crucial molecule synthesized by RNA polymerase from DNA. It serves various functions in the cell, including protein-coding and non-coding roles. Understanding the different types of RNA is essential for comprehending their specific functions. Let’s delve into the main types … Read more

Pulse Field Gel Electrophoresis (PFGE) Protocol

Pulse Field Gel Electrophoresis (PFGE) Protocol

Pulsed Field Gel Electrophoresis (PFGE) is a highly effective genotyping technique used for the separation and analysis of large DNA molecules, such as entire genomic DNA. It involves digesting the DNA with specific restriction enzymes and applying it to a gel matrix under an electric field that periodically changes direction. PFGE is a modified version … Read more

DNA Sequencing – Definition, Principle, Steps, Types, Applications

DNA Sequencing - Definition, Principle, Steps, Types, Applications

What is DNA Sequencing? Definition of DNA Sequencing DNA sequencing refers to the techniques used to determine the order of the nucleotide bases adenine, guanine, cytosine, and thymine within a DNA molecule. Methods of DNA Sequencing DNA sequencing is a crucial technique used in various fields of biological research, allowing scientists to unravel the genetic … Read more

DNA Microarray Principle, Types and Steps involved in cDNA microarrays

DNA Microarray Principle, Types and Steps involved in cDNA microarrays

The DNA microarray technology is among the most effective technology that can offer an extremely high-throughput and precise overview of the whole transcriptome and genome, which lets scientists understand the molecular processes that underlie natural and malfunctioning biological processes. Microarray technology may accelerate the process of screening thousands of protein and DNA samples at once.

Mechanisms of Protein Synthesis Regulation in Prokaryotic Cells

Mechanisms of Protein Synthesis Regulation in Prokaryotic Cells

Protein synthesis regulation is the intricate process by which cells control the production of proteins, which are essential for nearly all biological functions. This process involves the precise regulation of gene expression to ensure the synthesis of specific proteins at the right time and in the right amounts. Protein synthesis regulation plays a vital role … Read more

Restriction Fragment Length Polymorphism (RFLP)

Restriction Fragment Length Polymorphism (RFLP)

What is Restriction Fragment Length Polymorphism (RFLP)? Principle of Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a powerful technique used in molecular biology to analyze genetic variations and identify differences in DNA sequences. This technique relies on the principle that the DNA fragments generated by the digestion of DNA with specific … Read more

Vector – Definition, Examples, Characteristics, Types, Applications, Limitations

DNA Polymerase - Definition, Mechanism, Structure, Types

What is a vector in biology? Vector Definition A vector is a DNA molecule or genetic material used to transfer specific genetic information between cells in molecular biology and genetic engineering. Characteristics Features of vectors The following outlines the key features and functions of vectors, providing insights into their design and effectiveness. Types of vectors … Read more

DNA Polymerase – Definition, Mechanism, Structure, Types

DNA Polymerase - Definition, Mechanism, Structure, Types

What is DNA Polymerase? DNA polymerases Definition DNA polymerases are enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, playing a crucial role in DNA replication and repair across all living organisms. Properties of DNA Polymerase DNA polymerases possess several important properties that contribute to their role in DNA replication. These properties ensure … Read more

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