What is Chargaff’s Rules? Definition of Chargaff’s Rules Chargaff’s Rules are two fundamental principles discovered by biochemist Erwin Chargaff, which dictate the base pairing in DNA. The first rule states that in DNA, the amount of adenine (A) is equal to thymine (T), and the amount of cytosine (C) is equal to guanine (G). The … Read more
A dihybrid crossing is a gene-cross between people who have homozygous or heterozygous genotypes that have two distinct characteristics or traits. Dihybrid crosses differ from the monohybrid crossing that only involves one genetic character or characteristic.
What is DNA polymerase II? Definition of DNA polymerase II DNA polymerase II (DNA Pol II) is a prokaryotic DNA-dependent DNA polymerase that primarily functions as a backup enzyme in DNA replication and repair processes. It belongs to the B family of DNA polymerases and possesses DNA synthesis and proofreading activities. Structure of DNA polymerase … Read more
What is DNA polymerase III (holoenzyme)? Definition of DNA polymerase III (holoenzyme) DNA polymerase III holoenzyme is the primary enzyme complex in prokaryotes responsible for DNA replication, possessing both synthesis and proofreading capabilities to ensure accurate replication of the genome. Components of DNA polymerase III (holoenzyme) The DNA polymerase III holoenzyme, a central player in … Read more
What is DNA polymerase IV? Definition of DNA polymerase IV DNA polymerase IV is a prokaryotic enzyme encoded by the dinB gene, involved in mutagenesis and DNA repair processes, especially during the SOS response to DNA damage. Unlike many polymerases, it lacks proofreading activity, making it error-prone, but it plays a crucial role in translesion … Read more
What is Deletion Mutation? Definition of Deletion Mutation A deletion mutation is a genetic anomaly in which a segment of a chromosome or DNA sequence is omitted during DNA replication, leading to the absence of specific nucleotides or entire chromosomal segments. This can result in altered gene function or expression. Causes of Deletion Mutation Deletion … Read more
What is Interphase? Definition of Interphase Interphase is the phase in the eukaryotic cell cycle during which the cell grows, replicates its DNA, and prepares for cell division, encompassing the G1, S, and G2 sub-stages. Stages of Interphase 1. Gap 1 2. Synthesis In summation, the Synthesis phase is a critical juncture in the cell … Read more
What is Genetic Engineering? Definition of Genetic Engineering Genetic engineering is the deliberate manipulation of an organism’s DNA using advanced techniques to modify, add, or remove specific genes, resulting in desired genetic traits or functions. Basic Tools of Genetic Engineering Genetic engineering involves the manipulation and transfer of genes from one organism to another. To … Read more
What is DNA polymerase I? Definition of DNA polymerase I DNA polymerase I (Pol I) is an enzyme found in prokaryotes that plays a role in DNA replication and repair, primarily assisting in the removal of RNA primers and filling in the resulting gaps with DNA nucleotides. Structure of DNA polymerase I Mechanism of DNA … Read more
What is Recombinant DNA? Definition of Recombinant DNA Recombinant DNA (rDNA) is a form of DNA constructed in the laboratory by joining genetic material from multiple sources, creating sequences not naturally found in any organism. Characterisitcs Features of Recombinant DNA Recombinant DNA (rDNA) refers to the formation of new combinations of DNA sequences that are … Read more
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