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What is Gene cloning? “DNA Cloning” is a molecular biology technique that is utilized to create exact copies, or clones, of one particular gene or DN.” History of Gene cloning Principle of Gene Cloning Requirements for Gene Cloning Restriction Enzyme Digestion and Ligation Enzyme Source Recognition Sequence Restriction Digest EcoRI Escherichia coli 5’GAATTC3’CTTAAG 5′—G↓AATTC—3’3′—CTTAA↑G—5′ … Read more
In molecular biology and genetics, blotting is an analysis technique used for the detection of specific biomolecules (proteins, DNA or RNA) in samples of complex composition by transferring them onto a carrier such as a nitrocellulose, polyvinylidene fluoride or nylon membrane.
What is Hemoglobin? What is Globin Chain? The globin chain refers to the specific combination of protein subunits that make up different types of hemoglobin. Hemoglobin is composed of four globin chains, which can vary depending on the specific type of hemoglobin. The different types of hemoglobin and their corresponding globin chains are as follows: … Read more
What is Karyotyping? Definition of Karyotyping Karyotyping is a laboratory technique used to visualize and analyze the number, size, and shape of chromosomes in an individual’s cells, aiding in the detection of genetic abnormalities and chromosomal disorders. What is Idiogram? An idiogram is a diagrammatic representation of a karyotype, which showcases all the morphological features … Read more
What is Chargaff’s Rules? Definition of Chargaff’s Rules Chargaff’s Rules are two fundamental principles discovered by biochemist Erwin Chargaff, which dictate the base pairing in DNA. The first rule states that in DNA, the amount of adenine (A) is equal to thymine (T), and the amount of cytosine (C) is equal to guanine (G). The … Read more
A dihybrid crossing is a gene-cross between people who have homozygous or heterozygous genotypes that have two distinct characteristics or traits. Dihybrid crosses differ from the monohybrid crossing that only involves one genetic character or characteristic.
What is DNA polymerase II? Definition of DNA polymerase II DNA polymerase II (DNA Pol II) is a prokaryotic DNA-dependent DNA polymerase that primarily functions as a backup enzyme in DNA replication and repair processes. It belongs to the B family of DNA polymerases and possesses DNA synthesis and proofreading activities. Structure of DNA polymerase … Read more
What is DNA polymerase III (holoenzyme)? Definition of DNA polymerase III (holoenzyme) DNA polymerase III holoenzyme is the primary enzyme complex in prokaryotes responsible for DNA replication, possessing both synthesis and proofreading capabilities to ensure accurate replication of the genome. Components of DNA polymerase III (holoenzyme) The DNA polymerase III holoenzyme, a central player in … Read more
What is DNA polymerase IV? Definition of DNA polymerase IV DNA polymerase IV is a prokaryotic enzyme encoded by the dinB gene, involved in mutagenesis and DNA repair processes, especially during the SOS response to DNA damage. Unlike many polymerases, it lacks proofreading activity, making it error-prone, but it plays a crucial role in translesion … Read more
What is Deletion Mutation? Definition of Deletion Mutation A deletion mutation is a genetic anomaly in which a segment of a chromosome or DNA sequence is omitted during DNA replication, leading to the absence of specific nucleotides or entire chromosomal segments. This can result in altered gene function or expression. Causes of Deletion Mutation Deletion … Read more
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