CLED Agar Composition

Cystine-Lactose-Electrolyte-Deficient (CLED) medium, first described by Sandys and later modified by Mackey and Sandys, is generally used for diagnostic routine urinary bacteriology as a non-selective medium capable of supporting the growth of most urinary pathogens. CLED Agar, a differential medium for the isolation and counting of bacteria from urine, is used. It supports the growth all potential urinary pathogens. The medium also provides distinct colony morphology. It is suitable for the growth of all urinary pathogens, contaminants, and provides good colonial differentiation. However, it does not allow for the spread of Proteus species because of its low electrolytes.