SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

By BNO Team Published on May 15, 2024 2 min readby BNO Team

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SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	175.3 g	3 M
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol)	27.6 g	0.2 M
EDTA (mw: 292.24 g/mol)	7.4 g	0.02 M

SSPE (Blot, 20x, pH 7.4) Preparation

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 175.3 g of Sodium chloride to the solution.
3. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
4. Add 7.4 g of EDTA to the solution.
5. Add distilled water until the volume is 1 L.
6. Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.

SSC (Northern Blot, 20x) Preparation and Recipe

Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	175.3 g	3 M
Trisodium citrate (mw: 258.07 g/mol)	88.3 g	0.3 M

SSC (Northern Blot, 20x) Preparation

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 175.3 g of Sodium chloride to the solution.
3. Add 88.3 g of Trisodium citrate to the solution.
4. Add distilled water until the volume is 1 L.
5. Adjust the pH to 7.0 with NaOH or HCl. Autoclave the solution and store it at room temperature. It will last for 1 yr or longer.

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