SSPE (Blot, 20x, pH 7.4) Preparation and Recipe
Required components
| Component | Amount | Concentration |
| Sodium chloride (mw: 58.44 g/mol) | 175.3 g | 3 M |
| Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) | 27.6 g | 0.2 M |
| EDTA (mw: 292.24 g/mol) | 7.4 g | 0.02 M |
SSPE (Blot, 20x, pH 7.4) Preparation
- Prepare 800 mL of distilled water in a suitable container.
- Add 175.3 g of Sodium chloride to the solution.
- Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
- Add 7.4 g of EDTA to the solution.
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.
SSC (Northern Blot, 20x) Preparation and Recipe
Required components
| Component | Amount | Concentration |
| Sodium chloride (mw: 58.44 g/mol) | 175.3 g | 3 M |
| Trisodium citrate (mw: 258.07 g/mol) | 88.3 g | 0.3 M |
SSC (Northern Blot, 20x) Preparation
- Prepare 800 mL of distilled water in a suitable container.
- Add 175.3 g of Sodium chloride to the solution.
- Add 88.3 g of Trisodium citrate to the solution.
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.0 with NaOH or HCl. Autoclave the solution and store it at room temperature. It will last for 1 yr or longer.
Cite this post:
BNO Team. (2024, May 15). SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe. Biology Notes Online. Retrieved from https://biologynotesonline.com/ssc-northern-blot-20x-and-sspe-blot-20x-ph-7-4-preparation-and-recipe/
BNO Team. "SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe." Biology Notes Online, 15 May 2024, biologynotesonline.com/ssc-northern-blot-20x-and-sspe-blot-20x-ph-7-4-preparation-and-recipe/.
BNO Team. "SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe." Biology Notes Online (blog). May 15, 2024. https://biologynotesonline.com/ssc-northern-blot-20x-and-sspe-blot-20x-ph-7-4-preparation-and-recipe/.
