Biotechnology

Match the promoters listed in Group I with the tissues listed in Group II Group I: P. α-Amylase Q. Glutenin R. Phaseollin S. Patatin Group II: 1. Endosperm 2. Tuber 3. Aleurone 4. Cotyledon (A) P-3, Q-1, R-4, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-2, R-1, S-3 (D) P-1, Q-3, R-2, S-4

Consider the following statements. I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll. (A) only I is true (B) both I and II are true (C) only II is true (D) I is true … Read more

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means (A) E1 binds to its substrate with higher affinity than E2 (B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 … Read more

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. … Read more

Match the following antibiotics in Group I with their mode of action in Group II Group I: P. Chloramphenicol Q. Norfloxacin R. Puromycin S. Rifampicin Group II: 1. Binds to DNA gyrase 2. Binds to RNA Polymerase 3. Inhibits peptidyl transferase 4. Mimics aminoacyl-(tRNA** (A) P-1, Q-3, R-2, S-4 (B) P-3, Q-1, R-2, S-4 (C) … Read more

Match the chemicals in Group I with the possible type/class in Group II Group I: P. Picloram Q. Zeatin R. Thiamine S. Glutamine Group II: 1. Vitamin 2. Auxin 3. Amino Acid 4. Cytokinin (A) P-2, Q-4, R-1, S-3 (B) P-4, Q-1, R-2, S-3 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-2, R-1, S-3

Q.4 Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is (A) Streptomycin (B) Ampicillin (C) Vancomycin (D) Kanamycin

During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is (A) 2 ATP and 2 NADH (B) 2 ATP and 0 NADH (C) 4 ATP and 2 NADH (D) 4 ATP and 0 NADH

Q.5 Program used for essentially local similarity search is (A) BLAST (B) RasMol (C) ExPASY (D) SWISS-PROT

Identify the enzyme that catalyzes the following reaction: α-Ketoglutarate + NADH + NH4+ + H+ → Glutamate + NAD+ + H2O (A) Glutamate synthetase (B) Glutamate oxoglutarate aminotransferase (C) Glutamate dehydrogenase (D) α-ketoglutarate deaminase