Biotechnology

Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10⁸ cells / ml and the final acceptable contamination level is 10⁻³ cells, for how long should 1m³ medium be treated at temperature of 120° (thermal deactivation rate constant = 0.23 / min) … Read more

True breeding Drosophila flies with curved wings and dark bodies were mated with true breeding short wings and tan body Drosophila. The F1 progeny was observed to be with curved wings and tan body. The F1 progeny was again allowed to breed and produced flies of the following phenotype, 45 curved wings tan body, 15 … Read more

Match Group I with Group II Group I: P. Real Time-PCR Q. 2-D Electrophoresis R. Affinity chromatography S. Microarray Group II: 1. Biochips 2. Syber Green 3. Antibody linked sephrose beads 4. Ampholytes (A) P-1, Q-2, R-4, S-3 (B) P-2, Q-3, R-4, S-1 (C) P-2, Q-4, R-3, S-1 (D) P-3, Q-2, R-1, S-4

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. … Read more

Match the following antibiotics in Group I with their mode of action in Group II Group I: P. Chloramphenicol Q. Norfloxacin R. Puromycin S. Rifampicin Group II: 1. Binds to DNA gyrase 2. Binds to RNA Polymerase 3. Inhibits peptidyl transferase 4. Mimics aminoacyl-(tRNA** (A) P-1, Q-3, R-2, S-4 (B) P-3, Q-1, R-2, S-4 (C) … Read more

Match the chemicals in Group I with the possible type/class in Group II Group I: P. Picloram Q. Zeatin R. Thiamine S. Glutamine Group II: 1. Vitamin 2. Auxin 3. Amino Acid 4. Cytokinin (A) P-2, Q-4, R-1, S-3 (B) P-4, Q-1, R-2, S-3 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-2, R-1, S-3

Somatic cell gene transfer is used for P. transgenic animal production Q. transgenic diploid cell production R. classical breeding of farm animals S. in-vitro fertilization (A) P, R and S (B) P, Q and R (C) P and R (D) P only

Q.1 Hybridoma technology is used to produce (A) monoclonal antibodies (B) polyclonal antibodies (C) both monoclonal and polyclonal antibodies (D) B cells

Accession number is a unique identification assigned to a (A) single database entry for DNA/Protein (B) single database entry for DNA only (C) single database entry for Protein only (D) multiple database entry for DNA/Protein

Q.2 Ames test is used to determine (A) the mutagenicity of a chemical (B) carcinogenicity of a chemical (C) both mutagenicity and carcinogenicity of a chemical (D) toxicity of a chemical