Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand … Read more
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A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ … Read more
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Match the products in Group I with the microbial cultures in Group II used for their industrial production Group I: P. Gluconic acid Q. L-Lysine R. Dextran S. Cellulase Group II: 1. Leuconostoc mesenteroids 2. Aspergillus niger 3. Brevibacterium flavum 4. Trichoderma reesei (A) P-2, Q-1, R-3, S-4 (B) P-1, Q-3, R-4, S-2 (C) P-2, … Read more
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Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. … Read more
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Match the following antibiotics in Group I with their mode of action in Group II Group I: P. Chloramphenicol Q. Norfloxacin R. Puromycin S. Rifampicin Group II: 1. Binds to DNA gyrase 2. Binds to RNA Polymerase 3. Inhibits peptidyl transferase 4. Mimics aminoacyl-(tRNA** (A) P-1, Q-3, R-2, S-4 (B) P-3, Q-1, R-2, S-4 (C) … Read more
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Match the chemicals in Group I with the possible type/class in Group II Group I: P. Picloram Q. Zeatin R. Thiamine S. Glutamine Group II: 1. Vitamin 2. Auxin 3. Amino Acid 4. Cytokinin (A) P-2, Q-4, R-1, S-3 (B) P-4, Q-1, R-2, S-3 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-2, R-1, S-3
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Interferon-β is produced by (A) bacteria infected cells (B) virus infected cells (C) both virus and bacteria infected cells (D) fungi infected cells
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A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10^5 anchorage dependent cells per cm^2, the increase in the … Read more
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A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is (A) 0.009 (B) 0.027 (C) 0.9 (D) 0.27
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A neonatally thymectomized mouse, immunized with protein antigen shows (A) both primary and secondary responses to the antigen (B) only primary response to the antigen (C) delayed type hypersensitive reactions (D) no response to the antigen
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