Biotechnology

Match Group I with Group II Group I: P. Real Time-PCR Q. 2-D Electrophoresis R. Affinity chromatography S. Microarray Group II: 1. Biochips 2. Syber Green 3. Antibody linked sephrose beads 4. Ampholytes (A) P-1, Q-2, R-4, S-3 (B) P-2, Q-3, R-4, S-1 (C) P-2, Q-4, R-3, S-1 (D) P-3, Q-2, R-1, S-4

Match Group I with Group II Group I: P. Fibronectin Q. Insulin R. α-Macroglobulin S. Transferrin Group II: 1. Uptake of amino acids and glucose 2. Trypsin inhibitor 3. Binds iron 4. Cell attachment to substratum (A) P-2, Q-1, R-4, S-3 (B) P-3, Q-2, R-1, S-4 (C) P-4, Q-2, R-1, S-3 (D) P-4, Q-1, R-2, … Read more

Match the promoters listed in Group I with the tissues listed in Group II Group I: P. α-Amylase Q. Glutenin R. Phaseollin S. Patatin Group II: 1. Endosperm 2. Tuber 3. Aleurone 4. Cotyledon (A) P-3, Q-1, R-4, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-2, R-1, S-3 (D) P-1, Q-3, R-2, S-4

Consider the following statements. I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll. (A) only I is true (B) both I and II are true (C) only II is true (D) I is true … Read more

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means (A) E1 binds to its substrate with higher affinity than E2 (B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 … Read more

Match the items in Group I with Group II Group I (Vectors): P. λ phage Q. Bacterial Artificial Chromosomes (BACs) R. P1 derived Artificial Chromosomes (PACs) S. λ cosmid Group II (Maximum DNA packaging): 1. 35-45 kb 2. 100-300 kb 3. ≤ 300 kb 4. 5 – 25 kb (A) P-3, Q-4, R-1, S-2 (B) … Read more

Match Group I with Group II Group I: P. Staphylococcus aureus Q. Candida albicans R. Mycobacterium tuberculosis S. Lactobacillus lactis Group II: 1. Biofilms 2. Bacteriocins 3. Methicillin resistance 4. Isoniazid (A) P-1, Q-4, R-2, S-3 (B) P-2, Q-3, R-1, S-4 (C) P-3, Q-1, R-4, S-2 (D) P-1, Q-2, R-4, S-3

A mutant Gα protein with increased GTPase activity would (A) not bind to GTP (B) not bind to GDP (C) show increased signaling (D) show decreased signaling

Dizygotic twins are connected to a single placenta during their embryonic development. These twins (A) have identical MHC haplotypes (B) have identical TH cells (C) have identical T cells (D) can accept grafts from each other (both (A) and (B))

The dissociation constant Kd for ligand binding to the receptor is 10⁻⁷ M. The concentration of ligand required for occupying 10% of receptors is (A) 10⁻⁶ M (B) 10⁻⁷ M (C) 10⁻⁸ M (D) 10⁻⁹ M