Biotechnology

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means (A) E1 binds to its substrate with higher affinity than E2 (B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 … Read more

Match the items in Group I with Group II Group I (Vectors): P. λ phage Q. Bacterial Artificial Chromosomes (BACs) R. P1 derived Artificial Chromosomes (PACs) S. λ cosmid Group II (Maximum DNA packaging): 1. 35-45 kb 2. 100-300 kb 3. ≤ 300 kb 4. 5 – 25 kb (A) P-3, Q-4, R-1, S-2 (B) … Read more

Match Group I with Group II Group I: P. Staphylococcus aureus Q. Candida albicans R. Mycobacterium tuberculosis S. Lactobacillus lactis Group II: 1. Biofilms 2. Bacteriocins 3. Methicillin resistance 4. Isoniazid (A) P-1, Q-4, R-2, S-3 (B) P-2, Q-3, R-1, S-4 (C) P-3, Q-1, R-4, S-2 (D) P-1, Q-2, R-4, S-3

A mutant Gα protein with increased GTPase activity would (A) not bind to GTP (B) not bind to GDP (C) show increased signaling (D) show decreased signaling

Dizygotic twins are connected to a single placenta during their embryonic development. These twins (A) have identical MHC haplotypes (B) have identical TH cells (C) have identical T cells (D) can accept grafts from each other (both (A) and (B))

The dissociation constant Kd for ligand binding to the receptor is 10⁻⁷ M. The concentration of ligand required for occupying 10% of receptors is (A) 10⁻⁶ M (B) 10⁻⁷ M (C) 10⁻⁸ M (D) 10⁻⁹ M

Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand … Read more

A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ … Read more

Match the products in Group I with the microbial cultures in Group II used for their industrial production Group I: P. Gluconic acid Q. L-Lysine R. Dextran S. Cellulase Group II: 1. Leuconostoc mesenteroids 2. Aspergillus niger 3. Brevibacterium flavum 4. Trichoderma reesei (A) P-2, Q-1, R-3, S-4 (B) P-1, Q-3, R-4, S-2 (C) P-2, … Read more

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: Cytoplasmic male sterility (cms) is invariably due to defect(s) in mitochondrial function. Reason: cms can be overcome by pollinating a fertility restoring (Rf) plant with pollen from a non cms plant. (A) both (a) and (r) are true and (r) … Read more