Biotechnology

The mean value of acid concentration (A) 49.4 g/L (B) 48.0 g/L (C) 50.0 g/L (D) 47.5 g/L

The value of ΔG° given K’eq as 1.7 at 23°C will be (A) -17.19 kJ mol⁻¹ (B) -19.8 kJ mol⁻¹ (C) +52.82 kJ mol⁻¹ (D) -117.07 kJ mol⁻¹

The number of α-helical turns permeating the membrane Common Data: The width of the lipid bilayer membrane is 30 Å. It is permeated by a protein which is a right-handed α-helix. (A) 5.6 turns (B) 3.5 turns (C) 6.5 turns (D) 5.0 turns

The ΔG° for the oxidation of NADH by FAD Statement: The standard redox potential values for two half-reactions are given. NAD⁺ + H⁺ + 2e⁻ ↔ NADH -0.315 V FAD + 2H⁺ + 2e⁻ ↔ FADH₂ -0.219 V (A) -9.25 kJ mol⁻¹ (B) -103.04 kJ mol⁻¹ (C) +51.52 kJ mol⁻¹ (D) -18.5 kJ mol⁻¹   … Read more

The number of amino acid residues present in the protein Common Data: The width of the lipid bilayer membrane is 30 Å. It is permeated by a protein which is a right-handed α-helix. (A) 15 (B) 18 (C) 20 (D) 17

The flow rate required to result in a steady-state concentration of sucrose as 1.5 g/L in the bioreactor Common Data: A culture of Rhizobium is grown in a chemostat (100 m³) bioreactor. The feed contains 12 g/L sucrose, Ks for the organism is 0.2 g/L and μm = 0.3 h⁻¹. (A) 15 m³/h (B) 26 … Read more

If Yx/s = 0.4 g/g for the above culture and steady-state cell concentration in the bioreactor is 4 g/L, the resulting substrate concentration will be (A) 2 g/L (B) 8 g/L (C) 4 g/L (D) 6 g/L

The dissociation constant Kd for ligand binding to the receptor is 10⁻⁷ M. The concentration of ligand required for occupying 10% of receptors is (A) 10⁻⁶ M (B) 10⁻⁷ M (C) 10⁻⁸ M (D) 10⁻⁹ M

Receptor R is over expressed in CHO cells and analysed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm, the average number of receptors R per cell is (assume complete saturation of receptors with ligand … Read more

A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell? (A) 4.50 × 10⁵ (B) 3.50 × 10⁵ … Read more