Biotechnology

Protein-protein interactions are studied by: P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry Options: (A) P and S only (B) Q and S only (C) P and R only (D) Q and R only

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r): Assertion: Isopropylthiogalactoside (IPTG) is a gratuitous inducer of lactose operon. Reason: Gratuitous inducers are chemical analogs which behave like natural inducers but they do not serve as substrate for the enzymes that are subsequently synthesized. Options: (A) both (a) and (r) … Read more

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r): Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously. Reason: Synchronous culture could be obtained by starving cells for essential nutrient components. Options: (A) both (a) and (r) are true and (r) is … Read more

Which of the following characteristics with respect to bacterial DNA polymerase are TRUE? P. Initiation of chain synthesis Q. 5′-3′ polymerization R. 3′-5′ exonuclease activity S. 5′-3′ exonuclease activity Options: (A) P and Q only (B) Q and R only (C) Q and S only (D) P and S only

Maximum specific growth rate (Mmax) of a microorganism is calculated by taking the: (In=loge, X=biomass, t = time) Options: (A) slope of In X vs t of the growth cycle (B) slope of In X vs t during the exponential growth phase (C) slope of X vs t (D) slope of X vs t during … Read more

Identify the CORRECT statements: P. 5′ and 3′ ends of the transcripts can be mapped by utilizing polymerase chain reaction Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid R. T4 polynucleotide kinase is used for labeling 3′ end of DNA S. Baculovirus (Autographa californica) can be used as an insect expression … Read more

Value of the determinant mentioned below is: Matrix=​14021​01101​−12−121​​ Options: (A) 24 (B) -30 (C) -24 (D) -10

HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following: I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine. II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack … Read more

What will be the probability of having a colour-blind son to a woman with phenotypically normal parents and a colour-blind brother, and married to a normal man? (Assume that she has no previous children) Options: (A) 100 % (B) 50 % (C) 25 % (D) 12.5 %

Determine the correctness of the following statements: I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication. II. Enhancer sequences work by binding with eukaryotic gene activator factors. Options: (A) only I is true (B) only II is true (C) both I and II are true (D) both … Read more