Biotechnology

Q.1 Hybridoma technology is used to produce (A) monoclonal antibodies (B) polyclonal antibodies (C) both monoclonal and polyclonal antibodies (D) B cells

Accession number is a unique identification assigned to a (A) single database entry for DNA/Protein (B) single database entry for DNA only (C) single database entry for Protein only (D) multiple database entry for DNA/Protein

Q.2 Ames test is used to determine (A) the mutagenicity of a chemical (B) carcinogenicity of a chemical (C) both mutagenicity and carcinogenicity of a chemical (D) toxicity of a chemical

Expressed Sequence Tag is defined as (A) a partial sequence of a codon randomly selected from cDNA library (B) the characteristic gene expressed in the cell (C) the protein coding DNA sequence of a gene (D) uncharacterized fragment of DNA present in the cell

Q.3 The bacteria known to be naturally competent for transformation of DNA is (A) Escherichia coli (B) Bacillus subtilis (C) Mycobacterium tuberculosis (D) Yersinia pestis

In a chemostat operating under steady state, a bacterial culture can be grown at a dilution rate higher than maximum growth rate by (A) partial cell recycling (B) using sub-optimal temperature (C) pH cycling (D) substrate feed rate cycling

Q.4 Antibiotic resistance marker that CANNOT be used in a cloning vector in Gram negative bacteria is (A) Streptomycin (B) Ampicillin (C) Vancomycin (D) Kanamycin

During lactic acid fermentation, net yield of ATP and NADH per mole of glucose is (A) 2 ATP and 2 NADH (B) 2 ATP and 0 NADH (C) 4 ATP and 2 NADH (D) 4 ATP and 0 NADH

Q.5 Program used for essentially local similarity search is (A) BLAST (B) RasMol (C) ExPASY (D) SWISS-PROT

Identify the enzyme that catalyzes the following reaction: α-Ketoglutarate + NADH + NH4+ + H+ → Glutamate + NAD+ + H2O (A) Glutamate synthetase (B) Glutamate oxoglutarate aminotransferase (C) Glutamate dehydrogenase (D) α-ketoglutarate deaminase