Biotechnology

Q.5 Program used for essentially local similarity search is (A) BLAST (B) RasMol (C) ExPASY (D) SWISS-PROT

Identify the enzyme that catalyzes the following reaction: α-Ketoglutarate + NADH + NH4+ + H+ → Glutamate + NAD+ + H2O (A) Glutamate synthetase (B) Glutamate oxoglutarate aminotransferase (C) Glutamate dehydrogenase (D) α-ketoglutarate deaminase

Q.6 Peptidyl transferase activity resides in (A) 16S rRNA (B) 23S rRNA (C) 5S rRNA (D) 28S rRNA

The degree of inhibition for an enzyme-catalyzed reaction at a particular inhibitor concentration is independent of the initial substrate concentration. The inhibition follows (A) competitive inhibition (B) mixed inhibition (C) un-competitive inhibition (D) non-competitive inhibition

Q.7 In transgenics, alterations in the sequence of nucleotide in genes are due to P. Substitution Q. Deletion R. Insertion S. Rearrangement (A) P and Q (B) P, Q and R (C) Q and R (D) R and S

Oxidation-reduction reactions with positive standard redox potential (E⁰’) have (A) positive ΔG⁰ (B) negative ΔG⁰ (C) positive ΔE⁰’ (D) negative ΔE⁰’

Q.8 During transcription (A) DNA Gyrase introduces negative supercoils and DNA Topoisomerase I removes negative supercoils (B) DNA Topoisomerase I introduces negative supercoils and DNA Gyrase removes negative supercoils (C) both DNA Gyrase and DNA Topoisomerase I introduce negative supercoils (D) both DNA Gyrase and DNA Topoisomerase I remove negative supercoils

Nuclease-hypersensitive sites in the chromosomes are sites that appear to be (A) H2 and H4 histone free (B) H1 and H2 histone free (C) H3 and H4 histone free (D) Nucleosome free

Q.9 Under stress conditions bacteria accumulate (A) ppGpp (Guanosine tetraphosphate) (B) pppGpp (Guanosine pentaphosphate) (C) both ppGpp and pppGpp (D) either ppGpp or pppGpp

The formation of peptide cross-links between adjacent glycan chains in cell wall synthesis is called (A) Transglycosylation (B) Autoglycosylation (C) Autopeptidation (D) Transpeptidation