Outline the advantages of genetic screening, using the examples of breast cancer (BRCA1 and BRCA2), Huntington’s disease and cystic fibrosis
AS and A Level Biology
Explain the advantages of using recombinant human proteins to treat disease, using the examples insulin, factor VIII and adenosine deaminase
Explain the advantages of using recombinant human proteins to treat disease, using the examples insulin, factor VIII and adenosine deaminase
Outline the benefits of using databases that provide information about nucleotide sequences of genes and genomes, and amino acid sequences of proteins and protein structures
Outline the benefits of using databases that provide information about nucleotide sequences of genes and genomes, and amino acid sequences of proteins and protein structures
Outline how microarrays are used in the analysis of genomes and in detecting mRNA in studies of gene expression
Outline how microarrays are used in the analysis of genomes and in detecting mRNA in studies of gene expression
Describe and explain how gel electrophoresis is used to separate DNA fragments of different lengths
Describe and explain how gel electrophoresis is used to separate DNA fragments of different lengths
Describe and explain the steps involved in the polymerase chain reaction (PCR) to clone and amplify DNA, including the role of Taq polymerase
Describe and explain the steps involved in the polymerase chain reaction (PCR) to clone and amplify DNA, including the role of Taq polymerase
Explain that gene editing is a form of genetic engineering involving the insertion, deletion or replacement of DNA at specific sites in the genome
Explain that gene editing is a form of genetic engineering involving the insertion, deletion or replacement of DNA at specific sites in the genome
Explain how gene expression may be confirmed by the use of marker genes coding for fluorescent products
Explain how gene expression may be confirmed by the use of marker genes coding for fluorescent products
Explain why a promoter may have to be transferred into an organism as well as the desired gene
Explain why a promoter may have to be transferred into an organism as well as the desired gene
Explain the roles of restriction endonucleases, DNA ligase, plasmids, DNA polymerase and reverse transcriptase in the transfer of a gene into an organism
Explain the roles of restriction endonucleases, DNA ligase, plasmids, DNA polymerase and reverse transcriptase in the transfer of a gene into an organism