Which statement about DNA sequencing is false? a. The method developed by Sanger in the 1970s involved chemical modifications of bases b. The method developed by Sanger in the 1970s involved breaking up the DNA molecule into numerous small fragments. C_ High-throughput sequencing is generally faster than Sanger sequencing: d_ none of these statements are false

Sourav PanSeptember 18, 2024

Answered

The false statement is:

b. The method developed by Sanger in the 1970s involved breaking up the DNA molecule into numerous small fragments.

Explanation:

• a. The method developed by Sanger in the 1970s involved chemical modifications of bases: This is true. Sanger sequencing used dideoxynucleotides (ddNTPs) to terminate DNA strand elongation, which are chemically modified versions of normal nucleotides.
• b. The method developed by Sanger in the 1970s involved breaking up the DNA molecule into numerous small fragments: This is false. The original Sanger sequencing method did not involve breaking up DNA into small fragments. Instead, it involved sequencing single DNA fragments, with the use of chain-terminating ddNTPs to produce a set of fragments of varying lengths.
• c. High-throughput sequencing is generally faster than Sanger sequencing: This is true. High-throughput sequencing technologies can process millions of DNA sequences simultaneously, making them faster and more efficient than the Sanger method, which sequences one fragment at a time.
• d. none of these statements are false: This is not correct because statement b is false.