Basic Microbiology 3 Views 1 Answers
Which one of the following double-stranded sequences will NOT be recognized by a Type IIP restriction endonuclease?
Which one of the following double-stranded sequences will NOT be recognized by a Type IIP restriction endonuclease?
(A) 5′–GGTACC–3′
3′–CCTAGG–5′
(B) 5′–GGATCC–3′
3′–CCTAGG–5′
(C) 5′–CATATG–3′
3′–GTATAC–5′
(D) 5′–GATTTC–3′
3′–CTAAAG–5′
Answer
Answer: (D) 5′–GATTTC–3′
3′–CTAAAG–5′
Explanation:
Type IIP restriction endonucleases recognize specific palindromic sequences. The sequence in option D is not palindromic and therefore will not be recognized by a Type IIP restriction endonuclease.
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