Molecular biology 10 Views 1 Answers

Sourav PanLv 9September 18, 2024
Which of the following is a mechanism used in next generation high throughput DNA sequencing? -Ion Torrent -3 Step Method -Chain Terminators -Sanger Sequencing
Which of the following is a mechanism used in next generation high throughput DNA sequencing?
-Ion Torrent
-3 Step Method
-Chain Terminators
-Sanger Sequencing
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Sourav PanLv 9May 15, 2025
The mechanism used in next-generation high-throughput DNA sequencing from the options provided is:
– Ion Torrent
Explanation:
- Ion Torrent: This technology is a type of next-generation sequencing that uses semiconductor-based sequencing to detect changes in pH as nucleotides are incorporated into the DNA strand. This method is known for its high-throughput capabilities and is distinct from traditional Sanger sequencing.
- 3 Step Method: This term is not a specific mechanism used in next-generation sequencing. It may refer to various procedural methods but is not a recognized technology or mechanism in high-throughput sequencing.
- Chain Terminators: This is a mechanism used in Sanger sequencing, not in next-generation sequencing. Chain terminators are used to terminate DNA synthesis at specific points, which helps in determining the sequence of DNA fragments.
- Sanger Sequencing: This is a traditional sequencing method that predates next-generation sequencing. It uses chain-terminating dideoxynucleotides to sequence DNA but is not considered high-throughput.
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