Where does glycolysis occur in eukaryotes?

In eukaryotic cells, glycolysis occurs in the cytoplasm. This is true for both plant and animal cells. The enzymes necessary for glycolysis are localized in the cytosol, and the process does not require mitochondria. Once glycolysis produces pyruvate, the next steps depend on oxygen availability (aerobic respiration in mitochondria or fermentation in the cytoplasm under anaerobic conditions).