Where does DNA replication occur?

In eukaryotic cells, DNA replication occurs in the nucleus. The nucleus houses the cell’s genetic material and serves as the site where the DNA is unwound, replicated, and re-coiled for mitosis. In prokaryotic cells, which lack a membrane-bound nucleus, DNA replication takes place in the cytoplasm. The cell’s single circular chromosome is replicated in a process that begins at specific locations called origins of replication. Despite the location differences, the basic mechanisms of DNA replication are conserved across cell types.