What takes RNA primer detach in DNA replication?

The RNA primer is removed in DNA replication by a combination of enzymes. First, DNA polymerase I (in prokaryotes) or RNase H (in eukaryotes) recognizes the RNA-DNA hybrid at the primer and removes the RNA primer. DNA polymerase then fills in the gap left by the removed primer by synthesizing the correct DNA sequence. In prokaryotes, DNA polymerase I has both exonuclease activity to remove the primer and polymerase activity to replace it with DNA. In eukaryotes, RNase H removes the RNA primer, and DNA polymerase δ replaces it with DNA. Finally, DNA ligase seals the gap between the newly synthesized DNA and the existing DNA strand, completing the process. This ensures that the newly synthesized DNA is fully continuous, with no RNA left in the final strand.