What is the purpose of ampicillin in bacterial transformation?

Sourav PanDecember 7, 2024

Answered

Ampicillin is used in bacterial transformation as a selective agent to identify successfully transformed cells. Plasmids introduced during transformation often contain an ampicillin-resistance gene (bla), which allows transformed bacteria to grow in the presence of the antibiotic. Bacteria that fail to take up the plasmid are unable to survive on ampicillin-containing media, ensuring that only transformed cells are selected for further analysis.

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