What is biosafety level 2?

Biosafety Level 2 (BSL-2) is a classification for laboratories that handle moderate-risk biological agents capable of causing human disease. These agents are typically not transmitted via aerosols and are often indigenous to the community.

BSL-2 laboratories are suitable for work involving agents that pose moderate hazards to personnel and the environment. Examples of such agents include Hepatitis B virus, HIV, Salmonella spp., and Staphylococcus aureus.

The importance of BSL-2 lies in its role in enabling safe research and diagnostic activities involving moderate-risk pathogens. By implementing specific safety measures, BSL-2 laboratories protect laboratory personnel, the environment, and the surrounding community from potential exposure to infectious agents.

Historically, the concept of biosafety levels was developed to standardize laboratory practices and containment measures based on the risk associated with handling various biological agents. BSL-2 was established to address the need for enhanced safety protocols when working with agents that are more hazardous than those handled at BSL-1.

Key characteristics of BSL-2 laboratories include:

Access is restricted to trained personnel.

Use of personal protective equipment (PPE) such as lab coats, gloves, and eye protection.

Implementation of biosafety practices, including proper hand hygiene and decontamination procedures.

Availability of biological safety cabinets (Class II) for procedures that may generate aerosols.

Proper waste disposal methods, including autoclaving of biohazardous waste.

BSL-2 laboratories are commonly found in clinical, diagnostic, teaching, and research settings where work with moderate-risk agents is conducted. They play a crucial role in advancing scientific knowledge while ensuring the safety of laboratory personnel and the environment.