Answered
Limitations include:
• Limited duration: The system maintains anaerobic conditions only for a set period, which might not be sufficient for very slow-growing species.
• Capacity restrictions: The size of the jar limits the number of samples that can be incubated simultaneously.
• Seal dependency: The effectiveness relies on a perfect seal; any leaks can compromise the anaerobic environment.
• Sensitivity for fastidious organisms: Extremely oxygen-sensitive microbes might not survive even minimal residual oxygen.
• Limited environmental control: There is little flexibility to adjust the gas composition or temperature after the jar is sealed.
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