What are the limitations of the Beer-Lambert Law?

Sourav PanApril 24, 2025

Answered

The Beer-Lambert Law has several limitations:
– Non-linear behavior at high concentrations due to solute-solute interactions.
– Deviation from linearity at high absorbance values due to instrumental limitations.
– Applicability primarily to monochromatic light; deviations occur with polychromatic light.
– Assumes no scattering or fluorescence, which may not hold in all cases.
– Requires a homogeneous medium; inhomogeneous samples can lead to inaccuracies.
– Limited validity in complex matrices where chemical or physical changes occur.