Questions and Answers Archive - Page 61 of 87

Match the items in Group I with Group II Group I (Vectors): P. λ phage Q. Bacterial Artificial Chromosomes (BACs) R. P1 derived Artificial Chromosomes (PACs) S. λ cosmid Group II (Maximum DNA packaging): 1. 35-45 kb 2. 100-300 kb 3. ≤ 300 kb 4. 5 – 25 kb (A) P-3, Q-4, R-1, S-2 (B) P-1, Q-3, R-2, S-4 (C) P-4, Q-3, R-2, S-1 (D) P-1, Q-2, R-3, S-4

Match the items in Group I with Group II Group I (Vectors): P. λ phage Q. Bacterial Artificial Chromosomes (BACs) R. P1 derived Artificial Chromosomes (PACs) S. λ cosmid Group II (Maximum DNA packaging): 1. 35-45 kb 2. 100-300 kb 3. ≤ 300 kb 4. 5 – 25 kb (A) P-3, Q-4, R-1, S-2 (B) … Read more

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means (A) E1 binds to its substrate with higher affinity than E2 (B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 times that of E1 (C) The velocity of E1 catalyzed reaction is always greater than that of E2 (D) The velocity of E1 catalyzed reaction at a particular enzyme concentration and saturating substrate concentration is lower than that of E2 catalyzed reaction under the same conditions

The turnover numbers for the enzymes, E1 and E2 are 150 s⁻¹ and 15 s⁻¹ respectively. This means (A) E1 binds to its substrate with higher affinity than E2 (B) The velocity of reactions catalyzed by E1 and E2 at their respective saturating substrate concentrations could be equal, if concentration of E2 used is 10 … Read more

Consider the following statements. I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll. (A) only I is true (B) both I and II are true (C) only II is true (D) I is true and II is false

Consider the following statements. I. T4 DNA ligase can catalyze blunt end ligation more efficiently than E.coli DNA ligase II. The ligation efficiency of T4 DNA ligase can be increased with PEG and ficoll. (A) only I is true (B) both I and II are true (C) only II is true (D) I is true … Read more

Match the promoters listed in Group I with the tissues listed in Group II Group I: P. α-Amylase Q. Glutenin R. Phaseollin S. Patatin Group II: 1. Endosperm 2. Tuber 3. Aleurone 4. Cotyledon (A) P-3, Q-1, R-4, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-2, R-1, S-3 (D) P-1, Q-3, R-2, S-4

Match the promoters listed in Group I with the tissues listed in Group II Group I: P. α-Amylase Q. Glutenin R. Phaseollin S. Patatin Group II: 1. Endosperm 2. Tuber 3. Aleurone 4. Cotyledon (A) P-3, Q-1, R-4, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-2, R-1, S-3 (D) P-1, Q-3, R-2, S-4

Match Group I with Group II Group I: P. Fibronectin Q. Insulin R. α-Macroglobulin S. Transferrin Group II: 1. Uptake of amino acids and glucose 2. Trypsin inhibitor 3. Binds iron 4. Cell attachment to substratum (A) P-2, Q-1, R-4, S-3 (B) P-3, Q-2, R-1, S-4 (C) P-4, Q-2, R-1, S-3 (D) P-4, Q-1, R-2, S-3

Match Group I with Group II Group I: P. Fibronectin Q. Insulin R. α-Macroglobulin S. Transferrin Group II: 1. Uptake of amino acids and glucose 2. Trypsin inhibitor 3. Binds iron 4. Cell attachment to substratum (A) P-2, Q-1, R-4, S-3 (B) P-3, Q-2, R-1, S-4 (C) P-4, Q-2, R-1, S-3 (D) P-4, Q-1, R-2, … Read more

Match the chemicals in Group I with the possible type/class in Group II Group I: P. Picloram Q. Zeatin R. Thiamine S. Glutamine Group II: 1. Vitamin 2. Auxin 3. Amino Acid 4. Cytokinin (A) P-2, Q-4, R-1, S-3 (B) P-4, Q-1, R-2, S-3 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-2, R-1, S-3

Match the chemicals in Group I with the possible type/class in Group II Group I: P. Picloram Q. Zeatin R. Thiamine S. Glutamine Group II: 1. Vitamin 2. Auxin 3. Amino Acid 4. Cytokinin (A) P-2, Q-4, R-1, S-3 (B) P-4, Q-1, R-2, S-3 (C) P-3, Q-1, R-2, S-4 (D) P-4, Q-2, R-1, S-3

Match the following antibiotics in Group I with their mode of action in Group II Group I: P. Chloramphenicol Q. Norfloxacin R. Puromycin S. Rifampicin Group II: 1. Binds to DNA gyrase 2. Binds to RNA Polymerase 3. Inhibits peptidyl transferase 4. Mimics aminoacyl-(tRNA** (A) P-1, Q-3, R-2, S-4 (B) P-3, Q-1, R-2, S-4 (C) P-3, Q-1, R-4, S-2 (D) P-4, Q-2, R-3, S-1

Match the following antibiotics in Group I with their mode of action in Group II Group I: P. Chloramphenicol Q. Norfloxacin R. Puromycin S. Rifampicin Group II: 1. Binds to DNA gyrase 2. Binds to RNA Polymerase 3. Inhibits peptidyl transferase 4. Mimics aminoacyl-(tRNA** (A) P-1, Q-3, R-2, S-4 (B) P-3, Q-1, R-2, S-4 (C) … Read more

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. (A) both (a) and (r) are true and (r) is the correct reason for (a) (B) both (a) and (r) are true and (r) is not the correct reason for (a) (C) (a) is true but (r) is false (D) (a) is false but (r) is true

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: MTT assay is used to determine cell viability based on the principle of colour formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the colour development by converting tetrazolium soluble salt to insoluble salt. … Read more

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10^5 anchorage dependent cells per cm^2, the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are (A) 1200 cm^2 and 12 x 10^7 cells (B) 600 cm^2 and 6 x 10^7 cells (C) 600 cm^2 and 8300 cells (D) 1200 cm^2 and 8300 cells

A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10^5 anchorage dependent cells per cm^2, the increase in the … Read more

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than 1 could be apparently due to (A) substrate inhibited kinetics with external pore diffusion limitation (B) internal pore diffusion limitation (C) sigmoidal kinetics (D) unstability of the enzyme

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than 1 could be apparently due to (A) substrate inhibited kinetics with external pore diffusion limitation (B) internal pore diffusion limitation (C) sigmoidal kinetics (D) unstability of the enzyme