Biochemistry 222 Views 1 Answers
If a 10 mM solution of a biomolecule in a cuvette of path length 10 mm absorbs 90% of the incident light at 280 nm, the molar extinction coefficient of the biomolecule at this wavelength is _____________ M⁻¹cm⁻¹.
If a 10 mM solution of a biomolecule in a cuvette of path length 10 mm absorbs 90% of the incident light at 280 nm, the molar extinction coefficient of the biomolecule at this wavelength is _____________ M⁻¹cm⁻¹.
Answer
Answer: 1042.59 M⁻¹cm⁻¹
Explanation:
Using Beer’s Law:
A = \epsilon \cdot c \cdot l
Where:
- A = \log \left(\frac{100}{10}\right) = 1
- c = 10 \times 10^{-3} \text{ M}
- l = 1 \text{ cm}
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