How to use the Henderson-Hasselbalch equation to make a buffer?
How to use the Henderson-Hasselbalch equation to make a buffer?
Cite this post:
Sourav Pan. (2025, April 24). How to use the Henderson-Hasselbalch equation to make a buffer?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/
Sourav Pan. "How to use the Henderson-Hasselbalch equation to make a buffer?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/.
Sourav Pan. "How to use the Henderson-Hasselbalch equation to make a buffer?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/.
– Select a weak acid with a pKa close to the desired pH of the buffer solution. – Use the Henderson-Hasselbalch equation: pH = pKa + log₁₀([A⁻]/[HA]) to determine the required ratio of conjugate base ([A⁻]) to weak acid ([HA]). – Calculate the necessary concentrations or amounts of the acid and its conjugate base to achieve this ratio. – Prepare the buffer by mixing the calculated amounts of the weak acid and its conjugate base in solution.
Cite this post:
Sourav Pan. (2025, April 24). How to use the Henderson-Hasselbalch equation to make a buffer?. Biology Notes Online. Retrieved from https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/
Sourav Pan. "How to use the Henderson-Hasselbalch equation to make a buffer?." Biology Notes Online, 24 April 2025, biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/.
Sourav Pan. "How to use the Henderson-Hasselbalch equation to make a buffer?." Biology Notes Online (blog). April 24, 2025. https://biologynotesonline.com/qa/how-to-use-the-henderson-hasselbalch-equation-to-make-a-buffer/.
