Biochemistry 2 Views 1 Answers
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Sourav PanApril 24, 2025

How to use the Henderson-Hasselbalch equation for pH indicators?

How to use the Henderson-Hasselbalch equation for pH indicators?

Sourav Pan
Sourav PanApril 24, 2025

Answered

Indicators change color over a specific pH range. Use the equation to determine the pH at which the ratio of the deprotonated to protonated form of the indicator is 1, i.e., when pH = pKa of the indicator. This is the midpoint of the color change range.

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