How to use a volumetric pipette?
How to use a volumetric pipette?
Answered
To use a volumetric pipette, begin by ensuring it is clean and in good condition. Attach a clean pipette bulb or pipetting device. Immerse the tip of the pipette into the liquid to be transferred. Draw the liquid slowly into the pipette, carefully filling it up to the calibration mark, which is typically near the neck of the pipette. The meniscus of the liquid should be aligned with this mark at eye level for accurate measurement. Once the liquid reaches the calibration mark, transfer it to the receiving vessel by gently expelling the liquid, ensuring that the pipette is emptied completely. Because volumetric pipettes are designed for specific, fixed volumes, they offer very high precision and are typically used in applications like preparing solutions or performing titrations.