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Sourav PanDecember 1, 2024

How to use a volumetric pipette?

How to use a volumetric pipette?

Sourav Pan
Sourav PanDecember 1, 2024

Answered

Using a volumetric pipette involves a few steps to ensure precise measurement of a fixed volume. First, ensure that the pipette is clean and properly calibrated. Attach a pipette bulb or pipetting device to the pipette. Immerse the tip of the pipette into the liquid and use the suction device to draw the liquid up into the pipette. Carefully fill the pipette until the liquid reaches the calibration mark, which is usually located near the neck of the pipette. The meniscus should align with this mark at eye level to ensure accuracy. Once the pipette is filled to the correct volume, transfer the liquid by positioning the pipette tip over the receiving container and gently releasing the liquid by releasing the suction or pressing the plunger. Because volumetric pipettes are designed for a single fixed volume, they are highly accurate and typically used for precise measurements in tasks like titrations or preparing standard solutions.

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