Using a pipette involves several careful steps to ensure accurate liquid measurement and transfer. First, attach a clean pipette tip if necessary. When using a manual pipette, create suction by either squeezing a bulb or using the pipette’s plunger mechanism to draw the liquid into the pipette. Immerse the pipette tip into the liquid, ensuring it does not touch the sides of the container to avoid contamination. Once the liquid is drawn up, remove the pipette tip from the container and release the liquid by slowly expelling it into a receiving vessel, ensuring not to release it too quickly, as this can lead to inaccurate measurement. It is important to avoid air bubbles inside the pipette tip as they can skew the measurement. After use, dispose of disposable tips properly or clean reusable pipettes to prevent contamination.
