Answered
To use a pipette properly, start by selecting the appropriate pipette for the volume of liquid you wish to transfer. When using a manual pipette, attach the pipette tip securely to the end of the pipette. If using a mechanical pipette, ensure the device is properly set to the correct volume. For liquid transfer, create a vacuum by either pressing the pipette’s bulb or activating the plunger, and immerse the tip into the liquid you intend to transfer. Gradually release the vacuum to draw the liquid up into the pipette. Once the desired volume is inside, remove the pipette tip from the liquid and dispense the contents by expelling the liquid into a container, ensuring the pipette tip does not touch the sides of the container. When using a micropipette, a similar process occurs, but the liquid is drawn and dispensed through a push-button mechanism. Always make sure to release the plunger slowly to avoid drawing in excess liquid or introducing air bubbles, which can affect accuracy. After use, the pipette tip should be disposed of properly if it’s a disposable one, or the pipette should be cleaned thoroughly if it is reusable.
BNO Team