how to run polyacrylamide gel electrophoresis

Sourav PanFebruary 9, 2025

Answered

To run PAGE, samples are mixed with a loading buffer and loaded into the wells of the polymerized gel. The gel is submerged in an electrophoresis buffer, and an electric field is applied. Proteins or nucleic acids migrate through the gel based on size and charge. The process continues until separation is achieved, after which the gel is stained to visualize the bands.