Basic Microbiology 1 Views 1 Answers
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Sourav PanDecember 1, 2024

How to read pipettes?

How to read pipettes?

Sourav Pan
Sourav PanDecember 1, 2024

Answered

Reading a pipette accurately requires careful attention to the markings along its length. Most pipettes, including volumetric and graduated types, feature graduations that allow for precise measurement of liquids. To read a pipette, first ensure that the liquid inside is at rest and that the meniscus—the curve formed at the surface of the liquid—is clearly visible. The bottom of the meniscus should be aligned with the desired graduation mark on the pipette. For pipettes with multiple graduation marks (such as a serological pipette), reading is typically done at the bottom of the meniscus. It is important to ensure the pipette is held vertically when taking a reading to avoid parallax errors. For more precise measurements, certain pipettes (like micropipettes) are read digitally, with the volume displayed on a screen, eliminating the need for visual alignment.

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