How to read a serological pipette?
How to read a serological pipette?
Answered
Reading a serological pipette involves understanding its graduation marks and the meniscus of the liquid. Here are the steps: 1. Hold the pipette vertically, at eye level, to ensure accurate reading. 2. Look at the liquid in the pipette and find the meniscus, which is the curve formed at the surface of the liquid. The bottom of the meniscus should align with the graduation mark for accurate measurement. 3. The graduations are typically in milliliters (mL), and the liquid level should correspond with the nearest graduation. For larger volumes, the reading is more straightforward, but for smaller volumes, especially near the last graduation mark, care must be taken to ensure precision. 4. If dispensing liquid, ensure that the liquid is allowed to fully drain from the pipette tip, as residual liquid can affect accuracy.