Answered
Proper pipetting technique involves several key steps to ensure accuracy. First, select the appropriate pipette for your required volume and attach a clean, compatible pipette tip. Always pre-wet the tip by aspirating and dispensing the liquid once before performing the actual transfer to ensure consistent results. When aspirating, immerse the tip into the liquid at a slight angle to avoid air bubbles. Draw the liquid slowly into the pipette, ensuring that the meniscus aligns with the calibration mark for accurate measurement. To dispense the liquid, hold the pipette tip at the edge of the receiving vessel, then gently expel the liquid by pressing the plunger to the second stop. Ensure you expel all the liquid to avoid under-delivery. Lastly, dispose of the tip if it is disposable, or clean and calibrate the pipette for future use.
BNO Team