Biochemistry 4 Views 1 Answers

Sourav Pan🥇 GoldApril 24, 2025
How to prepare buffer using the Henderson-Hasselbalch equation?
How to prepare buffer using the Henderson-Hasselbalch equation?
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Sourav Pan🥇 GoldMay 15, 2025
1. Choose a weak acid with a pKa close to the desired pH. 2. Use the equation to calculate the required ratio of [A⁻]/[HA]. 3. Determine the total concentration needed for buffering capacity. 4. Calculate the individual concentrations of A⁻ and HA using the ratio and total concentration. 5. Prepare the solution by mixing the appropriate amounts of acid and conjugate base.
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