Answered
To pipette properly, start by selecting the appropriate pipette for the required volume and attaching a clean, suitable tip. For manual pipettes, press the plunger to the first stop, and immerse the tip into the liquid at an angle to avoid air bubbles. Slowly release the plunger to aspirate the liquid, ensuring that the liquid reaches the desired volume marked by the meniscus. When pipetting, ensure the liquid does not touch the sides of the container to avoid contamination. To dispense, position the pipette tip over the receiving container, press the plunger to expel the liquid, and allow any residual liquid to drain from the tip by waiting a moment before removing the pipette. Finally, after dispensing, dispose of the pipette tip if disposable or clean the pipette if reusable. Proper pipetting technique is crucial to ensuring accurate measurements and preventing contamination.
BNO Team