Basic Microbiology 1 Views 1 Answers
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Sourav PanDecember 1, 2024

How to pipette correctly?

How to pipette correctly?

Sourav Pan
Sourav PanDecember 1, 2024

Answered

To pipette correctly, ensure that you follow a precise procedure to avoid errors. Start by selecting the correct pipette for the required volume. Attach a clean, appropriate pipette tip. Before aspirating the liquid, pre-wet the pipette tip by drawing liquid into it and dispensing it back into the original container. This ensures accuracy and prevents the liquid from sticking to the walls of the tip. Immerse the tip into the liquid at a slight angle to avoid air bubbles and draw the liquid slowly into the pipette, ensuring that the meniscus is aligned with the desired volume marking. To dispense, position the pipette tip over the receiving vessel and expel the liquid fully. Never let the pipette tip touch the sides of the receiving container, as this can lead to contamination and inaccurate measurements. Finally, dispose of disposable tips or clean the pipette for reuse.

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