To use a pipette properly, begin by selecting the correct pipette for the volume of liquid you need to transfer. Attach a clean, disposable pipette tip to the end of the pipette if required. To draw liquid into the pipette, first create suction by either pressing a rubber bulb (for manual pipettes) or operating the pipette’s plunger (for mechanical pipettes). Immerse the pipette tip into the liquid, ensuring it does not touch the sides of the container to avoid contamination. Slowly release the suction mechanism to draw the liquid up into the pipette to the desired volume. Be sure to avoid air bubbles, which can affect accuracy. To dispense the liquid, place the tip over the receiving container and gently expel the liquid by pressing the plunger or releasing the suction. Make sure to deliver the entire volume without splashing or contaminating the tip. After use, dispose of the tip if it is disposable or clean the pipette thoroughly if reusable.
