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Autoclaving pipette tips involves subjecting them to high-pressure steam to sterilize and eliminate microbial contamination. To autoclave pipette tips, first ensure that the tips are in a safe, autoclave-compatible bag or tray. The tips should be placed in an upright position to allow the steam to penetrate evenly. The autoclave should be set to the standard sterilization cycle, typically 121°C (250°F) for 15 to 20 minutes. Avoid overloading the autoclave, as this can impede steam circulation and affect the sterilization process. After autoclaving, allow the tips to cool before handling to prevent burns or contamination from the sterile environment. Ensure that the pipette tips are made from materials suitable for autoclaving, typically polypropylene or certain other plastics.
BNO Team