Basic Microbiology 1 Views 1 Answers
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Sourav PanDecember 1, 2024

How do you use a pipette?

How do you use a pipette?

Sourav Pan
Sourav PanDecember 1, 2024

Answered

To use a pipette, begin by selecting the appropriate pipette for the desired volume. Attach a clean, disposable pipette tip if necessary. For manual pipettes, create suction by squeezing the pipette bulb or operating the plunger, depending on the pipette type. Immerse the tip of the pipette into the liquid, ensuring it does not touch the container’s sides to avoid contamination. Slowly release the suction mechanism to draw the liquid into the pipette, ensuring that the liquid reaches the desired volume as indicated by the meniscus. To dispense the liquid, position the pipette tip over the receiving container and gently expel the liquid by releasing the suction or pressing the plunger. After use, discard the tip (if disposable) or clean the pipette thoroughly if reusable.

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