Biochemistry 2 Views 1 Answers
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Sourav PanApril 24, 2025

Can the Henderson-Hasselbalch equation be used for bases?

Can the Henderson-Hasselbalch equation be used for bases?

Sourav Pan
Sourav PanApril 24, 2025

Answered

Yes, the Henderson-Hasselbalch equation can be adapted for basic buffers. For a buffer containing a weak base (B) and its conjugate acid (BH⁺), the equation is: pOH = pKb + log10([BH⁺]/[B]). Since pH + pOH = 14, it can also be expressed as: pH = 14 – pKb – log10([BH⁺]/[B]). This form is useful for calculating the pH of basic buffer solutions.

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