Preparation of Crystal Violet (Recipe for Crystal Violet)
This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.
Ingredients of Crystal Violet
| Ingredients | Amount |
| crystal violet | 2 g |
| 95% ethyl alcohol | 20 ml |
| ammonium citrate monohydrate | 0.8 g |
| deionized water | 80 ml |
Preparation Steps of Crystal Violet Stain
- Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
- Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
- Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.
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